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Status: Unchecked ×Keyword: protein symmetry ×Clear all

3 claims from 1 paper

1. Biochemistry, Genetics and Molecular Biology › Protein Structure and Dynamics

   Predicting the structure of large protein complexes using AlphaFold and Monte Carlo tree search

   Bryant, Pozzati, Zhu, Shenoy, Kundrotas and Elofsson · Nature Communications · 2022

   The authors predict large protein complexes by assembling AlphaFold-predicted subcomponents with Monte Carlo tree search, and add a scoring function, mpDockQ, to judge whether assemblies are complete and how accurate.

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   1. UncheckedUsing Monte Carlo tree search on predicted subcomponents, the authors assembled 91 of 175 complexes of 10–30 chains, with a median TM-score of 0.51.“We assemble 91 out of 175 complexes with 10–30 chains from predicted subcomponents using Monte Carlo tree search, with a median TM-score of 0.51.”
   2. UncheckedThe authors built a scoring function, mpDockQ, which they say can tell whether assembled protein complexes are complete and estimate how accurate they are.“We create a scoring function, mpDockQ, that can distinguish if assemblies are complete and predict their accuracy.”
   3. UncheckedIn this study, large protein complexes with symmetry were assembled accurately from predicted parts, while asymmetrical complexes remained difficult to assemble.“We find that complexes containing symmetry are accurately assembled, while asymmetrical complexes remain challenging.”